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Accessing CDS Data in SB-CGC
Seven Bridges Cancer Genomics Cloud (SB-CGC) is a cloud platform for the analysis and storage of large cancer datasets. There are two mechanisms for transferring HTAN data from the NCI's Cancer Data Service (CDS) to SB-CGC:
- Direct export from the CDS Portal.
- Export via a Data Repository Service (DRS) Manifest.
Access Requirements
SB-CGC Data access via Seven Bridges Cancer Genomics Cloud (SB-CGC) requires a CGC account [register here]. For further information on using SB-CGC resources including programmatic access options, you can explore their online documentation.
Access-controlled data To download access-controlled files, you must first complete a dbGAP request.
HTAN Data in CDS includes:
- Open Access Level 2 Imaging Files; and
- Access-controlled Sequencing Data (e.g. fastq and BAM files)
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Imaging Files
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Direct Export
In order to access HTAN imaging data within the CDS Portal, navigate to the portal in a web browser and click on the Explore CDS Data button on the landing page.
On the Data Explorer page, expand the STUDY section on the left sidebar, scroll down, and check the box next to Human Tumor Atlas (HTAN) imaging data.
This action will change the summary panel to reflect selecting HTAN data only.
Scroll down, or click on the Collapse View tab on the upper right just below the query summary line in order to see the tabulated view of all of the participants, samples or files in HTAN.
Click on the Add All Files button, or select the check boxes next to all Participants, Samples or Files for a subselection and then click on the Add Selected button. This action will update your cart icon in the upper right corner.
Clicking on the cart icon, will bring up a list of the selected files. Expand the Available Export Options drop down menu and select Export to Cancer Genomics Cloud.
Note: The Download Manifest can also be chosen instead of Export to Cancer Genomics Cloud. Please see
Follow the prompts to log in to CGC. Then select a Destination project, check the box to agree to CGC terms and import the data.
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DRS Manifest Files
DRS manifests are CSV files which list the files you would like to obtain. They require at minimum the name and drs_uri of each file of interest. For data transfer using a DRS Manifest, there are two main steps:
Generate the DRS Manifest Import the data to CGC
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1. Generate the DRS Manifest
For HTAN data, DRS Manifests can be generated from three different locations:
- CDS Portal
- HTAN Data Portal
- Google BigQuery
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Generating a DRS Manifest from the CDS Portal
Follow the directions for
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Generating a DRS Manifest from the HTAN Data Portal
From the HTAN Data Portal, click CDS/SB-CGC (Open Access) under the Data Access filter.
Navigate to the Files tab, check the box next to Filename in upper left, and then click Download selected files.
Click Download Manifest, which will download a local file called cds_manifest.csv
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Generating a DRS Manifest from Google BigQuery
HTAN metadata and a mapping of HTAN Data File IDs to CDS DRS URIs are available as Google BigQuery tables via the Institute for Systems Biology Cancer Gateway in the Cloud (ISB-CGC) (see Google BigQuery). These tables can be used to subset data to a cohort of interest, and obtain DRS URIs of files to access.
For a step-by-step guide on how to generate a DRS manifest file using Google BigQuery, please see the Python notebook Creating_CDS_Data_Import_Manifests_Using_BQ.ipynb.
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2. Import the data into CGC
Once you have your manifest, follow the instructions on SB-CGC's Import from a DRS server documentation page to import data from a manifest file.
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Sequencing Data
The CDS Portal, within NCI's Cancer Research Data Commons (CRDC), provides an interface to filter and select data from a variety of NCI programs, including controlled-access, primary sequence data from the Human Tumor Atlas Network (HTAN).
The directions for accessing sequencing data on CDS are similar to those for
- For Direct Export or Generating a DRS Manifest from CDS, choose Human Tumor Atlas (HTAN) primary sequence data on the STUDY section of the left hand sidebar instead of Human Tumor Atlas (HTAN) imaging data.
- To generate a DRS Manifest from the HTAN Data Portal, click CDS/SB-CGC (dbGaP) under the Data Access filter instead of CDS/SB-CGC (Open Access).